HPLC Method for Streptothricins, Streptomycin B, Streptomycin on Primesep 200 by SIELC Technologies
High Performance Liquid Chromatography (HPLC) Method for Analysis of Streptothricins, Streptomycin B, Streptomycin.
Streptomycin is an antibiotic used in treating bacterial infections and has also been used as a pesticide. It is on the World Health Organization’s (WHO) list of essential medicines. It works by inhibiting the synthesis of proteins. It can be retained on a Primesep 200 mixed-mode column by hydrophobic and ion-exchange properties. The analytical method uses MS-compatible mobile phase of acetonitrile (ACN) and water with sulfuric acid (H2SO4) buffer. Also capable of UV detection at 200nm.
| Column | Primesep 200 |
| Mobile Phase | MeCN/H2O – 10/90% |
| Buffer | H2SO4 – 0.05% |
| Flow Rate | 1.0 ml/min |
| Detection | UV, 200 nm |
| Class of Compounds |
Drug, Hydrophilic, Zwitterionic, Antibiotic |
| Analyzing Compounds | Streptothricins, Streptomycin B, Streptomycin |
Application Column
Primesep 200
Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended
Application Analytes:
StreptomycinStreptomycin B
Streptothricins
Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.
