HPLC Determination of Tranexamic acid (TXA) on Primesep 100 Column

HPLC Method for Analysis of Tranexamic acid on Primesep 100 by SIELC Technologies

Separation type: Liquid Chromatography Mixed-mode

HPLC Determination of  Tranexamic acid (TXA) on Primesep 100 Column
HPLC Method for Analysis of Tranexamic acid on Primesep 100 by SIELC Technologies

Tranexamic acid (TXA) is a synthetic derivative of the amino acid lysine. It is a medication used to treat or prevent excessive blood loss from major trauma, surgery, tooth removal, nosebleeds, and heavy menstruation. It is also used for hereditary angioedema. It can be taken by mouth, injected into a vein, or applied topically.

Tranexamic acid works by inhibiting the activation of plasminogen to plasmin, an enzyme that degrades fibrin clots, fibrinogen, and other proteins in the extracellular matrix. By inhibiting the activation of plasmin, tranexamic acid stabilizes clots and reduces bleeding.

Tranexamic acid can be retained on a Primesep 100 mixed-mode column with embedded acidic ion-pairing groups, having great peak shape using an isocratic method of acetonitrile (ACN), water and perchloric acid (HClO4) as a buffer. UV Detection 210nm.

High Performance Liquid Chromatography (HPLC) Method for Analysis of  Tranexamic acid

ColumnPrimesep 100, 4.6 x 150 mm, 5 µm, 100 A
Mobile PhaseMeCN/H2O – 20/80%
BufferHClO4 – 0.2%
Flow Rate1.0  ml/min
DetectionUV 210 nm
Class of Compounds
Acid, Drug
Analyzing CompoundsTranexamic acid

Application Column

Primesep 100

Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A

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Application Analytes:
Tranexamic acid

Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.