HPLC Determination of Uridine Diphospate Glucose on Newcrom BH

Separation type: Liquid Chromatography Mixed-mode




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High Performance Liquid Chromatography (HPLC) Method for Analysis of Fructose 1,6 Bisphosphate


Uridine Diphosphate Glucose (UDP-glucose) is an an important nucleotide sugar involved in metabolic processes in the cell. It is a precursor to glycogen, UDP-galactose, and UDP-glucuronic acid, as well as other polysacchrides and glycosphingolipids.
UDP-Galactose can be detected in the low UV regime. Using a Newcrom BH mixed-mode column and a mobile phase consisting of water and acetonitrile (MeCN) with a sulfuric acid (H2SO4) buffer, UDP-galactose can be retained, measured, and analyzed. This analysis method can be UV detected at 210 nm.




Condition
Column Newcron BH, 4.6×150 mm, 3 µm, 100A
Mobile Phase MeCN – 10%
Buffer H2SO4 – 0.2%
Flow Rate 1.0 ml/min
Detection UV 210 nm

 

Description
Class of Compounds Sugar, Phospate
Analyzing Compounds Uridine-5′-diphosphate-glucose

 

 

Application Column

Newcrom BH

The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.

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Application Analytes:
Uridine-5′-diphosphate-glucose
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.