HPLC Method for Analysis of Ascorbic Acid and Sodium Metabisulfite
Ascorbic is found naturally in citrus fruits and many vegetables. As a medication it is used to prevent or treat low levels of vitamin C, since it is that vitamin. Vitamin C is needed to maintain the health of skin, cartilage, teeth, bone, and blood vessels. Sodium metabisulfite is a white crystalline or powder solid. It has many uses, but some of it’s more prominent are: as the source of SO2 in wine, as a bleaching agent in the production of Coconut cream, and added to anaesthetic solutions to prevent oxidation to improve the shelf life of the solution. Primesep SB, a reverse-phase column, contains embedded basic ionizable groups and can retain Ascorbic acid and Sodium metabisulfite. The method is UV compatible and can be used as a general approach for analyzing similar compounds.