HPLC Method for Analysis of Ascorbic Acid and Sodium Metabisulfite

*The metabisulfite ion (S2O52−) is hydrolyzed to bisulfite (HSO3) in water. Sodium metabisulfite is a white crystalline or powder solid. It has many uses, but some of it’s more prominent are: as the source of SO2 in wine, as a bleaching agent in the production of Coconut cream, and added to anesthetic solutions to prevent oxidation to improve the shelf life of the solution. Ascorbic is found naturally in citrus fruits and many vegetables. As a medication, it is used to prevent or treat low levels of vitamin C, since it is that vitamin. Vitamin C is needed to maintain the health of skin, cartilage, teeth, bone, and blood vessels. Primesep SB, a reverse-phase column, contains embedded basic ionizable groups and can retain Ascorbic acid and Sodium bisulfite. The method is UV compatible and can be used as a general approach for analyzing similar compounds.



Column Primesep SB, 4.6x150 mm, 5 µm, 100A
Mobile Phase MeCN/H2O
Buffer H3PO4
Flow Rate 1.0 ml/min
Detection UV, 270 nm


Class of Compounds Acid, Hydrophilic, Ionizable, Disinfectant, Antioxidant, Preservative agent.
Analyzing Compounds Ascorbic Acid, Sodium Metabisulfite

Application Analytes:

Ascorbic Acid
Sodium bisulfite
Sodium metabisulfite