HPLC Method for Analysis of B-Estradiol on Primesep 200 Column

B-Estradiol is an estrogen steroid hormone. It is the major estrogen secreted by the premenopausal ovary. It is synthesized from testosterone primarily in the ovarian granulosa cells and placenta. B-estradiol is used as a medication, for instance in menopausal hormone therapy. Primesep 200, a reverse phase column, contains embedded acidic ionizable groups and can retain B-Estradiol. The method is UV compatible and can be used as a general approach for analyzing similar compounds.  


Column Primesep 200, 3.2x100 mm, 5 µm, 100A
Mobile Phase Gradient MeCN - 5-50%, 5 min, 4 min hold
Buffer Gradient H2SO4 - 0.05-0.3 %, 5 min, 4 min hold
Flow Rate 1.0 ml/min
Detection UV, 270 nm


Class of Compounds Drug, Hormone, Hydrophobic
Analyzing Compounds B-Estradiol

Application Analytes: