HPLC Method for Coumarin on Newcrom R1 by SIELC Technologies
High Performance Liquid Chromatography (HPLC) Method for Analysis of Coumarin
Coumarin is an aromatic chemical that is used as a fragrance ingredient. It has a sweet earthy scent and is typically found in Tonka beans, cinnamon, woodruff, bison grass, and green tea. Besides a pleasant smell it had anti-inflammatory, antibacterial, and antifungal properties.
Coumarin an be retained and analyzed using the Newcrom R1 stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with a phosphoric acid buffer. Detection is performed using UV.
| Column | Newcrom R1, 3.2 x 100 mm, 3 µm, 100 A, dual ended |
| Mobile Phase | MeCN/H2O – 25/75% |
| Buffer | H3PO4 – 0.1 % |
| Flow Rate | 0.5 ml/min |
| Detection | UV, 276 nm |
| Class of Compounds | Drug, Flavouring substance, Hydrophobic, Neutral |
| Analyzing Compounds | Coumarin |
Application Column
Newcrom R1
Column Diameter: 3.2 mm
Column Length: 100 mm
Particle Size: 3 µm
Pore Size: 100 A
Column options: dual ended
Application Analytes:
CoumarinApplication Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.
