HPLC Method for Analysis of Dyes in Walgreen’s Night Time Cough



Column Obelisc N, 3.26x50 mm, 5 µm, 100A
Mobile Phase MeCN/H2O - 50/50%
Buffer AmFm pH 3.0 - 70 mM
Flow Rate 0.6 ml/min
Detection UV, 630, 525 nm


Class of Compounds Dye, Acid, Hydrophilic, Ionizable, Zwitterionic
Analyzing Compounds Allura Red, Brilliant Blue

Application Analytes:

Allura Red
Brilliant Blue