HPLC Method for Analysis of Gabapentin Tablets

HPLC Method for Analysis of Gabapentin Tablets Gabapentin is a drug used to treat epilepsy, neuropathic pain, hot flashes, and restless legs syndrome. It also is used to treat neuropathic pain. Gabapentin can be analyzed by this reverse phase (RP) HPLC method with simple conditions. The mobile phase contains acetonitrile (MeCN), water, and phosphoric acid. For Mass-Spec (MS) compatible applications the phosphoric acid needs to be replaced with formic acid. Smaller 3 µm particle columns are available for fast UPLC applications. This liquid chromatography method is scalable and can be used for isolation of impurities in preparative separation. It is also suitable for pharmacokinetics.

Condition 1

Column Primesep 100, 4.6x150 mm, 5 µm, 100A
Mobile Phase MeCN/H2O - 30/70%
Buffer AmFm pH 3.0- 30 mM
Flow Rate 1.0 ml/min
Detection CAD (Corona) MS- compatible mobile phase
 

Condition 2

Column Primesep 100, 4.6x150 mm, 5 µm, 100A
Mobile Phase MeCN/H2O - 50/50%
Buffer H2SO4 - 0.05 %
Flow Rate 1.0 ml/min
Detection UV, 210 nm

Description

Class of Compounds Drug,  Nerve pain medication, Anticonvulsant, Hydrophobic, Ionizable, Zwitterionic
Analyzing Compounds Gabapentin
 

Application Analytes:

Gabapentin
Gabapentin hydrochloride