HPLC Method for Glycine methyl ester, Glycine on Primesep A by SIELC Technologies
High Performance Liquid Chromatography (HPLC) Method for Analysis of Glycine methyl ester, Glycine
Glycine Methyl Ester Hydrochloride is a water soluble organic compound that is typically used in synthesis of pharmaceuticals and pesticides. Besides that, it is also used as a flavor enhancer and nutritional supplement.
Glycine Methyl Ester Hydrochloride can be retained and analyzed using the Primesep-A stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with a sulfuric acid buffer. Detection is performed using UV.
Column | Primesep A, 3.2 x 150 mm, 5 µm, 100 A, dual ended |
Mobile Phase | MeCN/H2O – 10/90% |
Buffer | H2SO4 – 0.05% |
Flow Rate | 0.05 ml/min |
Detection | UV, 200 nm |
Class of Compounds |
Amino acid, Drug, Acid, Hydrophilic, Ionizable, Vitamin, Supplements, Zwitterionic |
Analyzing Compounds | Glycine methyl ester, Glycine |
Application Column
Primesep A
Column Diameter: 3.2 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended
Glycine methyl ester