HPLC Method for Analysis of Glycine and Glycine Methyl Ester Hydrochloride

HPLC Method for Glycine methyl ester, Glycine on Primesep 100 by SIELC Technologies

HPLC Method for Analysis of Glycine Methyl Ester Hydrochloride _1146

High Performance Liquid Chromatography (HPLC) Method for Analysis of Glycine methyl ester, Glycine 

Glycine and glycine methyl ester hydrochloride are important amino acid compounds widely used in pharmaceutical, biochemical, and peptide research. Both compounds are water-soluble and play critical roles in protein synthesis, peptide modification, and metabolic studies. High-performance liquid chromatography (HPLC) offers a precise and reliable method to separate, identify, and quantify glycine and its methyl ester derivative with excellent sensitivity and reproducibility. This analytical approach is essential for quality control, pharmaceutical formulation analysis, and research applications involving amino acid derivatives. Using HPLC for these compounds ensures accurate, consistent results in laboratory and industrial settings.

ColumnPrimesep 100, 4.6 x 150 mm, 5 µm, 100 A, dual ended
Mobile PhaseMeCN/H2O – 5/95%
BufferH3PO4 – 0.5%
Flow Rate1 ml/min
DetectionUV, 200 nm

Class of Compounds
 Amino acid, Drug, Acid, Hydrophilic, Ionizable, Vitamin, Supplements, Zwitterionic
Analyzing CompoundsGlycine methyl ester, Glycine

Application Column

Primesep 100

Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended

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Application Analytes:
Glycine
Glycine methyl ester
Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.