HPLC Method for Pantoprazole, Pantoprazole sodium on Newcrom R1 by SIELC Technologies
High Performance Liquid Chromatography (HPLC) Method for Analysis of Pantoprazole, Pantoprazole sodium
Pantoprazole is a proton pump inhibitor (PPI) with C16H15F2N3O4S molecular formula. It is used to decrease stomach acids amounts. It does that through binding to proton pumps in the stomach’s lining, therefore preventing the production of stomach acid.
Pantoprazole, Pantoprazole sodium can be retained and analyzed using the Newcrom R1 stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with a Triethanolamine buffer. Detection is performed using UV.
| Column | Newcrom R1, 4.6 x 100 mm, 5 µm, 100 A, dual ended |
| Mobile Phase | MeCN/H2O – 25/75% |
| Buffer | TEAPh – 10 mM |
| Flow Rate | 1.0ml/min |
| Detection | UV, 290 nm |
| Class of Compounds | Drug, Proton-pump inhibitor, Hydrophobic, Neutral |
| Analyzing Compounds | Pantoprazole, Pantoprazole sodium |
Application Column
Newcrom R1
Column Diameter: 4.6 mm
Column Length: 100 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended
Application Analytes:
PantoprazolePantoprazole sodium
Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.
