HPLC Method For Analysis Of  Patulin on Newcrom R1 Column

Separation type: Liquid Chromatography Reverse Phase.


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High Performance Liquid Chromatography (HPLC) Method for Analysis of Patulin.

Patulin is a toxic chemical contaminant produced by several species of mold, especially within Byssochlamys Aspergillus and Penicillium. It is the most common mycotoxin found in apples and apple-derived products such as juice, cider, compotes and other food intended for young children. Patulin can be retained in HPLC on Newcrom R1 reverse-phase column with the simple isocratic mobile phase consisting of acetonitrile (MeCN, ACN), water and Phosphoric Acid (H3PO4) buffer. The analysis method can be UV detected at 275 nm.

Condition

Column Newcrom R1, 3.2×100 mm, 5 µm, 100A
Mobile Phase MeCN – 10%
Buffer H3PO4 – 0.2%
Flow Rate 0.5 ml/min
UV Detection 275 nm
LOD 0.002 ppm

Description

Class of Compounds Hydrophilic
Analyzing Compounds Patulin

 

Application Column

Newcrom R1

The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.

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Application Analytes:
Patulin
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.