HPLC Method for Analysis of Dyes in Robitussin Cough + Chest

Sunset Yellow FCF, also known as Orange Yellow S, C.I. 15985, or Yellow 6, is a petroleum-derived orange azo dye. When in powder form, it is Reddish-orange, but when dissolved in water, the liquid becomes bright yellow. The dye is banned or restricted in Norway, Finland and Sweden. Obelisc N is a column with very polar characteristics. It contains embedded acidic and basic ionizable groups and can retain Sunset Yellow. The method is LC/MS and UV compatible and can be used as a general approach for analyzing similar compounds.  

Condition

Column Obelisc N, 3.2x50 mm, 5 µm, 100A
Mobile Phase MeCN/H2O - 50/50%
Buffer AmFm pH 3.0 - 70 mM
Flow Rate 0.6 ml/min
Detection UV, 525, 630 nm
 

Description

Class of Compounds Dye, Acid, Hydrophilic, Ionizable, Zwitterionic
Analyzing Compounds Allura Red, Brilliant Blue
 

Application Analytes:

HPLC Method for Analysis of Dyes in Robitussin Cough + Chest