HPLC Method for Analysis of Selenious Acid on Primesep B2 Column

Selenious Acid is used in protecting and changing the color of steel, most often in weaponry. It is highly toxic in excessive quantities, and if ingested, it could lead to death. The Acid is also capable of penetrating the skin and can produce acute poisonings. Primesep B2, a reverse-phase column, contains embedded basic ionizable groups and can retain Selenious Acid. The method is UV compatible and can be used as a general approach for analyzing similar compounds.  


Column Primesep B2, 4.6x50 mm, 5 µm, 100A
Mobile Phase MeCN/H2O - 50/50%
Buffer H3PO4 - 0.3%
Flow Rate 1.0 ml/min
Detection UV, 205 nm


Class of Compounds  Acid, Hydrophilic, Ionizable
Analyzing Compounds Selenious Acid

Application Analytes:

Selenious Acid