HPLC Method for Analysis of Sulfadiazine, Naphthylthiourea, Sulfamethazine, Dexamethasone, Cortisone 21-Acetate

Sulfadiazine is an antibiotic used to prevent rheumatic fever, chancroid, chlamydia and infections by Haemophilus influenzae. There are side effects to the use of the drug which include, but not limited to: nausea, headache, rash, depression.  

Condition

Column Primesep 100, 3.2x50 mm, 5 µm, 100A
Mobile Phase Gradient MeCN - 20-75%, 4 min
Buffer Gradient H3PO4 - 0.05-0.2%, 4 min
Flow Rate 0.5 ml/min
Detection UV, 270 nm
 

Description

Class of Compounds Drug, Acid, Hydrophilic, Ionizable
Analyzing Compounds Sulfadiazine, Naphthylthiourea, Sulfamethazine, Dexamethasone, Cortisone 21-Acetate
 

Application Analytes:

Cortisone 21-Acetate
Dexamethasone
Naphthylthiourea
Sulfadiazine
Sulfamethazine