HPLC Method for Analysis of Thiamine Pyrophosphate and Pyridoxal Phosphate


Column Primesep 100, 4.6x150 mm, 5 µm, 100A
Mobile Phase MeCN/H2O - 20/80%
Buffer AmFm pH 3.0- 10 mM
Flow Rate 1.0 ml/min
Detection UV, 250 nm


Class of Compounds Hydrophilic, Ionizable
Analyzing Compounds Thiamine Pyrophosphate, Pyridoxal Phosphate

Application Analytes:

Pyridoxal Phosphate
Thiamine diphosphate