HPLC Method for Analysis of Sunset Yellow in Walgreen’s Daytime Non-Drowsy Cold & Flu

Sunset Yellow FCF, also known as Orange Yellow S, C.I. 15985, or Yellow 6, is a petroleum-derived orange azo dye. When in powder form, it is Reddish-orange, but when dissolved in water, the liquid becomes bright yellow.The dye is banned or restricted in Norway, Finland and Sweden. Obelisc N is a column with very polar characteristics. It contains embedded acidic and basic ionizable groups and can retain Sunset Yellow. The method is LC/MS and UV compatible and can be used as a general approach for analyzing similar compounds.  


Column Obelisc N, 3.2x50 mm, 5 µm, 100A
Mobile Phase MeCN/H2O - 50/50%
Buffer AmFm pH 3.0 - 70 mM
Flow Rate 0.6 ml/min
Detection UV, 525 nm


Class of Compounds Dye, Acid, Hydrophilic, Ionizable, Zwitterionic
Analyzing Compounds Sunset Yellow

Application Analytes:

Sunset Yellow
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