HPLC Method of Ofloxacin in Ofloxacin 0.3 % Ear Drops on Primesep D Column

Separation type: Liquid Chromatography Mixed-mode


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High Performance Liquid Chromatography (HPLC) Method for Analysis of Ofloxacin

Ofloxacin is a common antibiotic most-often used to topically treat eye and ear infections via drops. Ofloxacin can be measured at low UV. Using a Primesep D reverse-phase column and a mobile phase consisting of water and an acetonitrile (MeCN) gradient with a sulfuric acid (H2SO4) buffer, Ofloxacin can be retained. This analysis method can be UV detected at 253 nm.

 

Condition 1

Column Primesep D, 4.6×150 mm, 5 µm, 100A
Mobile Phase Gradient MeCN – 10-20%, 10 min
Buffer H2SO4 – 0.1%
Flow Rate 1.0 mL/min
Detection UV 253 nm

 

Description

Class of Compounds
Antibiotics, Drug
Analyzing Compounds Ofloxacin

 

Application Column

Primesep D

The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.

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Application Analytes:
Ofloxacin
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.