HPLC Separation of β‑Hydroxy-β-methylbutyric Acid and 2-Hydroxyisocaproic Acid on Primesep 100 Colimn

HPLC Separation of β‑Hydroxy-β-methylbutyric Acid and 2-Hydroxyisocaproic Acid_1182
Both β-hydroxy-β-methylbutyric acid (HMB) and 2-hydroxyisocaproic acid (HICA) are metabolites of the essential dietary branched chain amino acid leucine.
 

Condition

Column Primesep 100, 4.6x150 mm, 5 µm, 100A
Mobile Phase MeCN - 5%
Buffer H2SO4 - 0.05 %
Flow Rate 1.0 ml/min
Detection UV, 210 nm
 

Description

Class of Compounds Acid, Hydrophilic,  Ionizable, Normal human metabolite, Dietary supplement, Essential amino acid, Branched-chain amino acid (BCAA)
Analyzing Compounds β‑Hydroxy-β-methylbutyric (HMB), 2-Hydroxyisocaproic Acid (HICA)
 


Application Analytes:

2-Hydroxyisocaproic Acid (HICA)
β‑Hydroxy-β-methylbutyric Acid (HMB)