HPLC Separation of Acrylic Acid and 4- Methoxyphenol on Primesep B Column

Acrylic acid in combination with other monomers produces copolymers which are used in manufacturing of various products such as paints, adhesives and plastics. Esters and salts of acrylic acid are known as acrylates. 4-methoxyphenol, also known as mequinol, is an active ingredient in topical drugs and is also used for inhibition of radical polymerization of monomers like acrylates. The two compounds can be separated on a mixed-mode Primesep B column with isocratic method in mobile phase of 20/80 Acetonitrile (ACN) and water with 0.02% phosphoric acid buffer. UV Detection 200nm.  
 

Condition

Column Primesep B, 4.6x150 mm, 5 µm, 100A
Mobile Phase MeCN/H2O - 20/80%
Buffer H3PO4 - 0.02 %
Flow Rate 1.0 ml/min
Detection UV, 200 nm
 

Description

Class of Compounds Acid, Hydrophilic, Ionizable, Antioxidant, Inhibitor, Phenol, Carboxylic acid
Analyzing Compounds  Acrylic Acid,  4- Methoxyphenol, Mequinol
 

Application Analytes:

4-Methoxyphenol
Acrylic acid