HPLC Separation of Catecholamines on Newcrom AH Column

Separation type: Liquid Chromatography Mixed-mode

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Condition

Column Newcrom AH, 4.6x150 mm, 5 µm, 100A
Mobile Phase H2O
Buffer H3PO4  or AmFm pH 3.0
Flow Rate 1.0 ml/min
Detection UV, 275 nm
 

Description

Class of Compounds Drug, Acid, Hydrophilic, Ionizable, Hormone
Analyzing Compounds  Norepinephrine, Epinephrine, Dopamine
 

Application Analytes:

Dopamine
Epinephrine
Norepinephrine