HPLC Method for Separation of Chlorhexidine and Cetylpyridinium Chloride on Primesep B Column

HPLC Separation of Chlorhexidine and Cetylpyridinium chloride on Primesep B Column_1222

Chlorhexidine gluconate, or simply chlorhexidine, is a biguanide used as an antiseptic and disinfectant. It is a component of mouthwash rinses that has been shown to reduce plaque, gingivitis and oral bacteria. It’s also used as a topical agent for skin disinfection. Cetylpyridinium chloride is another type of antiseptic used in mouthwash rinses. Both compounds are cationic. They can be separated using HPLC on SIELC’s reverse-phase (RP) mixed-mode Primesep B column with the mobile phase of acetonitrile (ACN) and water with formic acid buffer and UV detected at 270nm.

Condition

Column Primesep B,  3.2×150 mm, 5 µm, 100A
Mobile Phase MeCN/H2O
Buffer Formic Acid – 0.5%
Flow Rate 0.5 ml/min
Detection UV 270 nm

Description

Class of Compounds
Surfactant,  Hydrophobic, Ionizable
Analyzing Compounds Chlorhexidine, Cetylpyridinium Chloride

Application Column

Primesep B

The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.

Select options
Application Analytes:
Cetylpyridinium Chloride
Chlorhexidine
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.