HPLC Separation of Guanosine, Deoxyguanosine, Adenosine, Deoxyadenosine on Newcrom AH Column

Separation type: Liquid Chromatography Mixed-mode HPLC.cloud View on hplc.cloud Nucleosides are the building blocks for DNA and RNA as well as other roles in biomechanical processes such as signal transduction. By using a Newcrom AH mixed-mode column with a cation-exchange mechanism, nucleosides: guanosine, deoxyguanosine, adenosine, and deoxyadenosine, can be baseline separated in a short time using an isocratic method with a simple mobile phase of water, acetonitrile (MeCN, ACN), and H3PO4 as a buffer. UV detection at 210 nm.

Condition

Column Newcrom AH, 4.6x150 mm, 5 µm, 100A
Mobile Phase MeCN/H2O - 20/80%
Buffer H3PO4 - 0.5%
Flow Rate 1.0 ml/min
Detection UV, 210 nm
 

Description

Class of Compounds Nucleoside,  Hydrophilic, Ionizable
Analyzing Compounds Guanosine, Deoxyguanosine, Adenosine, Deoxyadenosine
 

Application Analytes:

Adenosine
Deoxyadenosine
Deoxyguanosine
Guanosine