HPLC Separation of Mixture of DMF and DMSO

Dimethylformamide, DMF, is primarily used as an organic solvent and is miscible with water and most other organic liquids. Dimethyl sulfoxide (DMSO) is also a solvent. It can dissolve both polar and nonpolar compounds and is miscible with water and other organic liquids. Both compounds can be retained and separated using anhydrous (water-free) conditions using HPLC by SHARC 1 column, which uses hydrogen-bonding as a separation mechanism. The method uses a gradient of acetonitrile (ACN) and methanol (MeOH) mobile phase without the need for a buffer. UV detection used at 220nm.


Column Sharc 1, 4.6x150 mm, 5 µm, 100A
Mobile Phase Gradient MeCN - 98-85 %, 10 min
Buffer No
Flow Rate 1.0 ml/min
Detection UV, 220 nm


Class of Compounds Drug, Basic, Hydrophobic, Ionizable, Zwitterionic
Analyzing Compounds DMF, DMSO

Application Analytes:

DMF (Dimethylformamide)
DMSO (Dimethyl sulfoxide)