HPLC Separation Of Mixture Of Pyridylacetic Acids (2-Pyridylacetic acid, 3-Pyridylacetic acid, 4-Pyridylacetic acid) On Primesep N Column

HPLC Method for 2-Pyridylacetic acid hydrochloride, 3-Pyridylacetic acid hydrochloride, 4-Pyridylacetic acid hydrochloride on Primesep N by SIELC Technologies

High Performance Liquid Chromatography (HPLC) Method for Analysis of 2-Pyridylacetic acid hydrochloride, 3-Pyridylacetic acid hydrochloride, 4-Pyridylacetic acid hydrochloride 

Pyridylacetic Acids are a class of compounds with a pyridine ring and an acetic acid. These acids are typically building blocks found in organic synthesis/ On occasion, they can also act as human xenobiotic metabolites.

2-Pyridylacetic acid hydrochloride, 3-Pyridylacetic acid hydrochloride, 4-Pyridylacetic acid hydrochloride can be retained and analyzed using the Primesep N stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with a ammonium formate buffer. Detection is performed using UV.

ColumnPrimesep N, 4.6 x 150 mm, 5 µm, 100 A, dual ended
Mobile PhaseMeCN/H2O – 80/20%
BufferAmFm pH 4.0- 20 mM
Flow Rate1.0 ml/min
DetectionUV, 270 nm
Class of Compounds
Acid, Hydrophilic, Ionizable,  Metabolite of nicotine and other tobacco alkaloids
Analyzing Compounds2-Pyridylacetic acid hydrochloride
3-Pyridylacetic acid hydrochloride
4-Pyridylacetic acid hydrochloride

Application Column

Primesep N

Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended

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Application Analytes:
2-Pyridylacetic acid hydrochloride
3-Pyridylacetic acid hydrochloride
4-Pyridylacetic acid hydrochloride
Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.