HPLC Separation of Nordihydrocapsaicin, Capsaicin and Dihydrocapsaicin

Separation type: Liquid Chromatography Reverse Phase




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High Performance Liquid Chromatography (HPLC) Method of Nordihydrocapsaicin Capsaicin and Dihydrocapsaicin.

Capsaicinoids (capsaicin, dihydrocapsaicin and nordihydrocapsaicin) are a group of phenolic alkaloids specific to the genus Capsicum and are comprised of a vanillylamine head and a fatty acid tail. Capsaicin, dihydrocapsaicin and nordihydrocapsaic can be retained and separated in HPLC using Newcrom R1 reverse-phase column isocratically. The method uses a simple MS-compatible mobile phase of acetonitrile (ACN) and water without the need for a buffer. The peaks can be monitored by low UV (210 nm), ELSD, CAD or LC/MS.



Condition

Column Newcrom R1, 3.2×100 mm, 5 µm, 100A
Mobile Phase MeCN/H2O – 45/55%
Buffer No
Flow Rate 0.5 ml/min
Detection 220 nm

Description

Class of Compounds Hydrophobic
Analyzing Compounds Nordihydrocapsaicin, Capsaicin,  Dihydrocapsaicin

 

Application Column

Newcrom R1

The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.

Select options
Application Analytes:
Capsaicin
Dihydrocapsaicin
Nordihydrocapsaicin
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.