HPLC Separation of Perfluoroalkyl Acids PFBA, PFAO, PEAS on Newcrom A Column

HPLC.cloud View on hplc.cloud Perfluoroalkyl acids (PFAAs) such as Perfluorobutyric acid, Perfluorononanoic acid and Perfluorooctylsulfonic acid are contaminants that have increasingly been found in groundwater and drinking water systems. Perfluoroalkyl acids (PFAAs) are also strong surfactants that have been used industrially, in the production of fluoropolymers, but they are also present as residual impurities in a wide range of polymeric consumer products e.g. used to impart water and stain repellency in textiles, papers, and boards. We were able to separate Perfluoroalkyl acids (PFAAs) in HPLC on Newcrom A mixed-mode column using a mobile phase of acetonitrile (ACN), and water with Ammonium Formate buffer. This separation can be monitored with ELSD, CAD detection techniques.


Column Newcom A, 4.6x150 mm, 5 µm, 100A
Mobile Phase MeCN - 50%
Buffer AmFm pH 3.0 – 50 mM
Flow Rate 1.0 ml/min
Detection CAD


Class of Compounds Perfluoroalkyl acids
Analyzing Compounds Perfluorobutyric acid, Perfluorononanoic acid, Perfluorooctylsulfonic acid

Application Analytes:

Perfluorobutyric Acid (PFBA)
Perfluorooctanoic acid (PFOA)
Perfluorooctylsulfonic acid (PFAS)