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Uracil, Thymine, Guanine, Cytosine and Adenine are the nucleobases found in RNA and DNA. The nucleobases are difficult to separate on reverse-phase columns due to their polar, hydrophilic and ionic nature. Using the Newcrom AH mixed-mode column, the nucleobases can be easily separated isocratically using low organic mobile phase (5% acetonitrile) or pure water, if organic mobile phase is undesirable, with ammonium formate buffer, making the method both UV and Mass Spec compatible.
| Column | Newcrom AH, 4.6x150 mm, 5 µm, 100A |
| Mobile Phase | MeCN/H2O - 5/95% |
| Buffer | AmFm pH 3.0- 30 mM |
| Flow Rate | 1.0 ml/min |
| Detection | UV 260 nm, MS-compatible mobile phase |
| Column | Newcrom AH, 4.6x150 mm, 5 µm, 100A |
| Mobile Phase | H2O - 100% |
| Buffer | AmFm pH 3.0- 10 mM |
| Flow Rate | 1.0 ml/min |
| Detection | UV 260 nm, MS-compatible mobile phase |
| Class of Compounds | Hydrophilic, Drug, Xanthine, Nucleic Bases |
| Analyzing Compounds | Uracil, Thymine, Guanine, Cytosine, Adenine |
Application Analytes:
Adenine
Cytosine
Guanosine
Thymine
Uracil