HPLC Separation of Uracil, Thymine, Guanine, Cytosine, Adenine on Newcrom AH

HPLC.cloud View on hplc.cloud HPLC Separation of Uracil, Thymine, Guanine, Cytosine, Adenine on Newcrom AH Column_1245 Uracil, Thymine, Guanine, Cytosine and Adenine are the nucleobases found in RNA and DNA.  The nucleobases are difficult to separate on reverse-phase columns due to their polar, hydrophilic and ionic nature.  Using the Newcrom AH mixed-mode column, the nucleobases can be easily separated isocratically using low organic mobile phase (5% acetonitrile) or pure water, if organic mobile phase is undesirable, with ammonium formate buffer, making the method both UV and Mass Spec compatible.

Condition 1

Column Newcrom AH, 4.6x150 mm, 5 µm, 100A
Mobile Phase MeCN/H2O - 5/95%
Buffer AmFm pH 3.0- 30 mM
Flow Rate 1.0 ml/min
Detection UV 260 nm,  MS-compatible mobile phase
 

Condition 2

Column Newcrom AH, 4.6x150 mm, 5 µm, 100A
Mobile Phase H2O - 100%
Buffer AmFm pH 3.0- 10 mM
Flow Rate 1.0 ml/min
Detection UV 260 nm,  MS-compatible mobile phase

Description

Class of Compounds Hydrophilic, Drug, Xanthine, Nucleic Bases
Analyzing Compounds Uracil, Thymine, Guanine, Cytosine, Adenine


Application Analytes:

Adenine
Cytosine
Guanine
Thymine
Uracil