Inosine is a nucleoside made of a hypoxanthine and a ribose ring. As a drug, Inosine is useful for the treatment of various autoimmune diseases. Legacy L1 was used to retain Inosine by reverse phase mechanism. Legacy L1 uses embedded C18 groups on porous silica and is useful for many USP HPLC applications. comparisons to Phenomenex columns are available by request.
HPLC Separation of Acedoben and Inosine on SHARC 1 Column
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Inosine pranobex is an antiviral drug. A combination of inosine and dimepranol acedoben (a salt of acetamidobenzoic acid and dimethylaminoisopropanol) has no effect on viral particles itself. Instead, it acts as an immunostimulant. It is most commonly used to treat the rare measles complication subacute sclerosing panencephalitis in conjunction with intrathecal interferon therapy.
Chromatography of these two compounds can be difficult due to their high polarity. But both compounds can be well retained and separated using anhydrous (water-free) conditions using HPLC on SHARC 1 column, which uses hydrogen-bonding as a separation mechanism. The method uses a gradient of acetonitrile (ACN) and methanol (MeOH) mobile phase with volatile buffer containing Formic Acid 0.1% and AmFm - 0.01%, making the method MS-compatible. Both compounds can also be UV detected at 270 nm.