L-Histidine hydrochloride monohydrate

CAS Number 5934-29-2
Molecular Formula C6H12ClN3O3
Molecular Weight 209.630 g/mol
InChI Key CMXXUDSWGMGYLZ-XRIGFGBMSA-N
LogP -2.01
Synonyms
  • L-Histidine hydrochloride monohydrate
  • L-Histidine--hydrogen chloride--water (1/1/1)
  • L-Histidine, hydrochloride, hydrate (1:1:1)
  • 5934-29-2
  • L-?Histidine, hydrochloride, hydrate (1:1:1)
  • Histidine, monohydrochloride, monohydrate, L-
  • L-Histidine, monohydrochloride, monohydrate
  • L-Histidine monohydrate monohydrochloride
  • HISTIDINE, L-, MONOHYDROCHLORIDE MONOHYDRATE
  • Histidine hydrochloride hydrate
  • L-Histidine HCl hydrate
  • UNII-X573657P6P
  • 35343-95-4

Applications:


Separation of L-Histidine hydrochloride monohydrate on Newcrom R1 HPLC column

L-Histidine hydrochloride monohydrate can be analyzed by this reverse phase (RP) HPLC method with simple conditions. The mobile phase contains an acetonitrile (MeCN), water, and phosphoric acid. For Mass-Spec (MS) compatible applications the phosphoric acid needs to be replaced with formic acid. Smaller 3 µm particles columns available for fast UPLC applications. This liquid chromatography method is scalable and can be used for isolation impurities in preparative separation. It also suitable for pharmacokinetics.

Separation of L-Histidine hydrochloride monohydrate on Newcrom C18 HPLC column
Newcrom R1

This column is available in new type of hardware with only one connection end. Learn more about performance and advantage of this new hardware.



Application Analytes:

L-Histidine hydrochloride monohydrate

HPLC Separation of Mixture of Essential Amino Acids on Primesep 100 Column
 

Condition

Column Primesep 100, 4.6x250 mm, 5 µm, 100A
Mobile Phase MeCN/H2O - 25/75%
Buffer Gradient H2SO4 0.05-0.2% 25 min
Flow Rate 1.0 ml/min
Detection UV, 200 nm
 

Description

Class of Compounds Drug, Acid, Hydrophilic, Ionizable, Vitamin, Supplements, Amino acid
Analyzing Compounds L-Threonine (Thr/T), L-Valine (Val/V), DL-Methionine (Met/M), L-Isoleucine (Ile/I), L-Leucine (Leu/L), L-Phenylalanine (Phe/F), L-Histidine (His/H), L- Tryptophan (Trp/W)
 

Application Analytes:

Amino Acids
D-Isoleucine
D-Leucine
D-Valine
DL-Isoleucine
Histidine
Isoleucine
L-Histidine hydrochloride monohydrate
L-Isoleucine
L-Methionine
L-Threonine
Methionine
Phenylalanine
Tryptophan
Valine

HPLC Method For Analysis Of Histidine and Histidine Methyl Ester Dihydrochloride on Primesep 100 Column
Separation type: Liquid Chromatography Mixed-mode

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High Performance Liquid Chromatography (HPLC) Method for Analysis of Histidine and Histidine Methyl Ester Dihydrochloride
Histidine and Histidine Methyl Ester Dyhydrochloride are related compounds with interesting uses. Histidine is used in biosynthesis to create proteins, while histidine methyl ester dihydrochloride (a derivative of histidine) is used to create various other compounds. This amino acid and its derivative can be detected in the low UV regime. Using a Primesep 100 mixed-mode column and a mobile phase consisting of water and acetonitrile (MeCN) with a sulfuric acid (H2SO4) buffer, Histidine and Histidine Methyl Ester Dyhydrochloride can be retained, separated, and analyzed. This analysis method can be UV detected at 200 nm.


Condition

Column Primesep 100, 4.6x150 mm, 5 µm, 100A
Mobile Phase MeCN/H2O - 60/40%
Buffer H2SO4 - 0.1%
Flow Rate 1.0 ml/min
Detection UV, 200 nm
 

Description

Class of Compounds Amino acid
Analyzing Compounds Histidine,  Histidine Methyl Ester
 

Application Analytes:

Histidine
Histidine methyl ester
L-Histidine hydrochloride monohydrate