|Molecular Weight||209.630 g/mol|
L-Histidine hydrochloride monohydrate can be analyzed by this reverse phase (RP) HPLC method with simple conditions. The mobile phase contains an acetonitrile (MeCN), water, and phosphoric acid. For Mass-Spec (MS) compatible applications the phosphoric acid needs to be replaced with formic acid. Smaller 3 µm particles columns available for fast UPLC applications. This liquid chromatography method is scalable and can be used for isolation impurities in preparative separation. It also suitable for pharmacokinetics.
This column is available in new type of hardware with only one connection end. Learn more about performance and advantage of this new hardware.
|Column||Primesep 100, 4.6x250 mm, 5 µm, 100A|
|Mobile Phase||MeCN/H2O - 25/75%|
|Buffer||Gradient H2SO4 0.05-0.2% 25 min|
|Flow Rate||1.0 ml/min|
|Detection||UV, 200 nm|
|Class of Compounds||Drug, Acid, Hydrophilic, Ionizable, Vitamin, Supplements, Amino acid|
|Analyzing Compounds||L-Threonine (Thr/T), L-Valine (Val/V), DL-Methionine (Met/M), L-Isoleucine (Ile/I), L-Leucine (Leu/L), L-Phenylalanine (Phe/F), L-Histidine (His/H), L- Tryptophan (Trp/W)|