L-Histidine hydrochloride monohydrate

CAS Number 5934-29-2
Molecular Formula C6H12ClN3O3
Molecular Weight 209.630 g/mol
InChI Key CMXXUDSWGMGYLZ-XRIGFGBMSA-N
LogP -2.01
Synonyms
  • L-Histidine hydrochloride monohydrate
  • L-Histidine--hydrogen chloride--water (1/1/1)
  • L-Histidine, hydrochloride, hydrate (1:1:1)
  • 5934-29-2
  • L-?Histidine, hydrochloride, hydrate (1:1:1)
  • Histidine, monohydrochloride, monohydrate, L-
  • L-Histidine, monohydrochloride, monohydrate
  • L-Histidine monohydrate monohydrochloride
  • HISTIDINE, L-, MONOHYDROCHLORIDE MONOHYDRATE
  • Histidine hydrochloride hydrate
  • L-Histidine HCl hydrate
  • UNII-X573657P6P
  • 35343-95-4

Applications:


Separation of L-Histidine hydrochloride monohydrate on Newcrom R1 HPLC column

L-Histidine hydrochloride monohydrate can be analyzed by this reverse phase (RP) HPLC method with simple conditions. The mobile phase contains an acetonitrile (MeCN), water, and phosphoric acid. For Mass-Spec (MS) compatible applications the phosphoric acid needs to be replaced with formic acid. Smaller 3 µm particles columns available for fast UPLC applications. This liquid chromatography method is scalable and can be used for isolation impurities in preparative separation. It also suitable for pharmacokinetics.

Separation of L-Histidine hydrochloride monohydrate on Newcrom C18 HPLC column
Newcrom R1

This column is available in new type of hardware with only one connection end. Learn more about performance and advantage of this new hardware.



Application Analytes:

L-Histidine hydrochloride monohydrate

HPLC Separation of Mixture of Essential Amino Acids on Primesep 100 Column
 

Condition

Column Primesep 100, 4.6x250 mm, 5 µm, 100A
Mobile Phase MeCN/H2O - 20/80%
Buffer Gradient H2SO4 0.05-0.2% 25 min
Flow Rate 1.0 ml/min
Detection UV, 200 nm
 

Description

Class of Compounds Drug, Acid, Hydrophilic, Ionizable, Vitamin, Supplements, Amino acid
Analyzing Compounds Amino acids
 

Application Analytes:

Amino Acids
D-Isoleucine
D-Leucine
D-Valine
DL-Isoleucine
Histidine
Isoleucine
L-Histidine hydrochloride monohydrate
L-Isoleucine
L-Methionine
Methionine
Phenylalanine
Tryptophan
Valine