| CAS Number | 13614-98-7 |
|---|---|
| Molecular Formula | C23H28ClN3O7 |
| Molecular Weight | 493.941 |
| InChI Key | GLMUAFMGXXHGLU-VQAITOIOSA-N |
| LogP | -0.139 |
| Synonyms |
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Applications:
HPLC Method For Analysis Of Minocycline on Primesep 100 column
August 9, 2021
Separation type: Liquid Chromatography Mixed-mode
High Performance Liquid Chromatography (HPLC) Method for Analysis of Minocycline hydrochloride
Minocycline is in a class of medications called tetracycline antibiotics. It works in treatment of infections by preventing the growth and spread of bacteria. Minocycline can be retained on the Primesep 100 mixed-mode column using an isocratic analytical method with a simple mobile phase of water, acetonitrile (MeCN, ACN), and sulphuric acid (H2SO4) buffer. The analysis method can be UV detected at 270 nm.
| Column | Primesep 100, 4.6×150 mm, 5 µm, 100A |
| Mobile Phase | MeCN/H2O – 50/50% |
| Buffer | H2SO4 – 0.5% |
| Flow Rate | 1.0 ml/min |
| Detection | UV, 270 nm |
| Class of Compounds |
Drug, Antibiotics |
| Analyzing Compounds | Minocycline hydrochloride |
Application Column
Primesep 100
The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.
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HPLC Method For Analysis Of Minocycline Hydrochloride on Newcrom R1 Column
May 22, 2018
HPLC Method for Minocycline hydrochloride on Newcrom R1 by SIELC Technologies
High Performance Liquid Chromatography (HPLC) Method for Analysis of Minocycline hydrochloride
Minocycline hydrochloride is a tetracycline antibiotic with C23H27N3O7 • HCl [XH2O] molecular formula. It is used to treat a wide variety of bacterial infections, acne, and sexually transmitted infections.
Minocycline hydrochloride can be retained and analyzed using the Newcrom R1 stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with a triethanolamine buffer. Detection is performed using UV.
| Column | Newcrom R1, 3.2 x 100 mm, 5 µm, 100 A, dual ended |
| Mobile Phase | MeCN/H2O – 5/95% |
| Buffer | TEAPh – 20 mM, pH 2.0 |
| Flow Rate | 0.5 ml/min |
| Detection | UV, 270 nm |
| Class of Compounds | Drug, Antibiotics |
| Analyzing Compounds | Minocycline hydrochloride |
Application Column
Newcrom R1
Column Diameter: 3.2 mm
Column Length: 100 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended
