Morphine

Morphine structural formula

CAS Number57-27-2
Molecular FormulaC17H19NO3
Molecular Weight285.343
InChI KeyBQJCRHHNABKAKU-KBQPJGBKSA-N
LogP0.89
Synonyms
  • Morphine
  • (5alpha,6alpha)-17-Methyl-7,8-didehydro-4,5-epoxymorphinan-3,6-diol
  • Morphinan-3,6-diol, 7,8-didehydro-4,5-epoxy-17-methyl-, (5alpha,6alpha)-
  • 57-27-2
  • MOR
  • Morphinan-3,6-diol, 7,8-didehydro-4,5-epoxy-17-methyl- (5?,6?)-
  • (-)-Morphine
  • Aguettant
  • DepoDur
  • Dulcontin
  • Duromorph
  • l-Morphine
  • Meconium
  • M-Eslon
  • morfina
  • Morphia
  • Morphin
  • Morphina
  • Morphinan-3,6-diol, 7,8-didehydro-4,5-epoxy-17-methyl-(5?,6?)-
  • Morphinan-3,6-diol, 7,8-didehydro-4,5-epoxy-17-methyl-, (5?,6?)-
  • Morphinan-3,6?-diol, 7,8-didehydro-4,5?-epoxy-17-methyl-
  • Morphinism
  • Morphinum
  • Morphium
  • MS Contin
  • Nepenthe
  • Ospalivina
  • Statex SR
  • Cube juice
  • 7,8-Didehydro-4,5-epoxy-17-methyl-morphinan-3,6-diol
  • Dreamer
  • EINECS 200-320-2
  • Hard stuff
  • 9H-9,9c-Iminoethanophenanthro(4,5-bcd)furan-3,5-diol, 4a,5,7a,8-tetrahydro-12-methyl-
  • Ms Emma
  • 4,5alpha-Epoxy-17-methyl-7-morphinen-3,6alpha-diol
  • UNII-76I7G6D29C
  • Sevredol
  • Dinamorf
  • (5R,6S,9R,13S,14R)-4,5-epoxy-N-methyl-7-morphinen-3,6-diol
  • (5alpha,6alpha)-17-methyl-7,8-didehydro-4,5-epoxymorphinan-3,6-diol
  • (5alpha,6alpha)-didehydro-4,5-epoxy-17-methylmorphinan-3,6-diol
  • 4-methyl-(13R,14S)-12-oxa-4-azapentacyclo[9.6.1.01,13.05,17.07,18]octadeca-7(18),8,10,15-tetraene-10,14-diol

Applications:

HPLC Separation of Morphine and Hydromorphone on Primesep 100 Column

August 3, 2021

Separation type: Liquid Chromatography Mixed-mode


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High Performance Liquid Chromatography (HPLC) Method for Analysis Morphine and Hydromorphone

Morphine and Hydromorphone are opioid medications. Morphine works by blocking pain signals from traveling along the nerves to the brain. Hydromorphone is a semisynthetic derivative of morphine. Morphine and Hydromorphone can be retained and separated on the Primesep 100 mixed-mode column using an isocratic analytical method with a simple mobile phase of water, acetonitrile (MeCN, ACN), and sulphuric acid (H2SO4) buffer. The analysis method can be UV detected at 200 nm.

Condition

Column Primesep 100,  4.6×150 mm, 5 µm, 100A
Mobile Phase MeCN/H2O – 30/70%
Buffer H2SO4 – 0.2%
Flow Rate 1.0  ml/min
Detection UV 200 nm

 

Description

Class of Compounds
 Drug
Analyzing Compounds Morphine and Hydromorphone

Application Column

Primesep 100

The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.

Select options
Application Analytes:
Hydromorphone
Morphine
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

Separation of Morphine on Newcrom R1 HPLC column

May 16, 2018
Separation of Morphine on Newcrom R1 HPLC column

Morphine is an opiate used to treat pain. It is extracted from opium poppies, specifically from the poppy straw. Morphine can be analyzed by this reverse phase (RP) HPLC method with simple conditions. The mobile phase contains acetonitrile (MeCN), water, and phosphoric acid. For Mass-Spec (MS) compatible applications the phosphoric acid needs to be replaced with formic acid. Smaller 3 µm particle columns are available for fast UPLC applications. This liquid chromatography method is scalable and can be used for isolation of impurities in preparative separation. It also suitable for pharmacokinetics.

Application Column

Newcrom R1

The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.

Select options
Application Analytes:
Morphine
The result was obtained by a proprietary SIELC algorithm. It may deviate from the actual experimental data. The experimental data are available upon request. Contact us by e-mail: support@sielc.com or by phone: 847-229-2629.