| CAS Number | 24280-93-1 |
|---|---|
| Molecular Formula | C17H20O6 |
| Molecular Weight | 320.342 |
| InChI Key | HPNSFSBZBAHARI-RUDMXATFSA-N |
| LogP | 3.40 |
| Synonyms |
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Applications:
HPLC Separation of Mixture of Mycophenolate mofetil and Mycophenolic Acid on Newcrom R1 Column
May 2, 2019
Mycophenolate mofetil and mycophenolic acid alongside other medicines are used as immunosuppressants to prevent organ rejection after a transplant. Both compounds can be retained and separated in HPLC using Newcrom R1 reverse-phase column. The analytical method’s mobile phase consists of a gradient of acetonitrile (ACN) in water with phosphoric acid (H3PO4) buffer and using UV detection at 220nm.
| Column | Newcrom R1, 4.6×150 mm, 5 µm, 100A |
| Mobile Phase | MeCN/H2O – 30/70% |
| Buffer | H3PO4- 0.2% |
| Flow Rate | 1.0 ml/min |
| Detection | UV, 220 nm |
| Class of Compounds |
Drug, Acid, Hydrophobic, Ionizable, Immunosuppressant |
| Analyzing Compounds | Mycophenolate mofetil, Mycophenolic Acid |
Application Column
Newcrom R1
The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.
Select optionsMycophenolic acid
HPLC Determination of Mycophenolic Acidon Primesep SB Column
May 1, 2019
Mycophenolic acid alongside other medicines is used as an immunosuppressant to prevent organ rejection after a transplant. THe compound can be retained in HPLC using Primesep SB mixed-mode column that has both hydrophobic and anion-exchange properties. The analytical method is isocratic and uses the mobile phase of acetonitrile (ACN) in water with phosphoric acid (H3PO4) buffer with UV detection at 226nm.
| Column | Primesep SB, 4.6×150 mm, 5 µm, 100A |
| Mobile Phase | MeCN/H2O – 40/60% |
| Buffer | H3PO4- 0.4% |
| Flow Rate | 1.0 ml/min |
| Detection | UV, 226 nm |
| Class of Compounds |
Drug, Acid, Hydrophobic, Ionizable, Immunosuppressant |
| Analyzing Compounds | Mycophenolic Acid |
Application Column
Primesep SB
The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.
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Separation of Mycophenolic acid on Newcrom R1 HPLC column
February 16, 2018
Mycophenolic acid can be analyzed by this reverse phase (RP) HPLC method with simple conditions. The mobile phase contains an acetonitrile (MeCN), water, and phosphoric acid. For Mass-Spec (MS) compatible applications the phosphoric acid needs to be replaced with formic acid. Smaller 3 µm particles columns available for fast UPLC applications. This liquid chromatography method is scalable and can be used for isolation impurities in preparative separation. It also suitable for pharmacokinetics.
Application Column
Newcrom R1
The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.
Select options