| CAS Number | 616-91-1 |
|---|---|
| Molecular Formula | C5H9NO3S |
| Molecular Weight | 163.190 |
| InChI Key | PWKSKIMOESPYIA-BYPYZUCNSA-N |
| LogP | -0.695 |
| Synonyms |
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Applications:
Determination N-Acetyl-L-Cysteine on Primesep SB Column
February 25, 2021
Separation type: Liquid Chromatography Mixed-mode
High Performance Liquid Chromatography (HPLC) Method of N-Acetyl-L-Cysteine.
N-Acetyl-L-Cysteine (NAC) is the N-acetyl derivative of the naturally occurring amino acid L-cysteine. It can be retained on Primesep SB, a reverse-phase (RP) mixed-mode column with embedded strong basic ion-pairing groups, using an isocratic analytical method with a simple mobile phase of water, acetonitrile (MeCN, ACN), and phosphoric acid (H3PO4) buffer. UV detection at 200 nm.
| Column | Primesep SB, 3.2×100 mm, 5 µm, 100A |
| Mobile Phase | MeCN – 20% |
| Buffer | H3PO4 0.3% |
| Flow Rate | 0.5 ml/min |
| Detection | UV, 200 nm |
| Column | Primesep SB, 3.2×100 mm, 5 µm, 100A |
| Mobile Phase | MeCN – 50% |
| Buffer | Na2HPO4 pH 4.0 – 50 mM |
| Flow Rate | 0.5 ml/min |
| Detection | UV, 200 nm |
| Class of Compounds |
Nutritional supplement, Hydrophilic, Ionizable |
| Analyzing Compounds | N-Acetyl-L-Cysteine |
Application Column
Primesep SB
The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.
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Separation of N-Acetyl-L-cysteine on Newcrom R1 HPLC column
February 16, 2018
N-Acetyl-L-cysteine can be analyzed by this reverse phase (RP) HPLC method with simple conditions. The mobile phase contains an acetonitrile (MeCN), water, and phosphoric acid. For Mass-Spec (MS) compatible applications the phosphoric acid needs to be replaced with formic acid. Smaller 3 µm particles columns available for fast UPLC applications. This liquid chromatography method is scalable and can be used for isolation impurities in preparative separation. It also suitable for pharmacokinetics.
Application Column
Newcrom R1
The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.
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