| Molecular Formula | C13H16N2O2 |
|---|---|
| Molecular Weight | 232.28 g/mol |
| InChI Key | SOSHNYKNINOSTB-LBPRGKRZSA-N |
| LogP | -0.1 |
| Synonyms |
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Applications:
HPLC Separation of Biogenic Amines on Primesep 100 Column
Tryptophan is an essential amino acid used in the synthesis of proteins. Tryptamine is structurally similar to tryptophan. Serotonin is a neurotransmitter derived from tryptophan. Dimethyltryptamine and 5-MeO-DMT are psychedelic substances derived from tryptamine. All the compounds have similar structures and can present difficulties to separation in reverse-phase HPLC. They can be separated using the Primesep 100 mixed-mode column in gradient analysis with acetonitrile (ACN) and water mobile phase with ammonium formate (AmFm) buffer, making the method MS-compatible. The amines can also be UV detected at 280nm.
| Column | Primesep 100, 4.6x150 mm, 5 µm, 100A |
| Mobile Phase | Gradient MeCN - 40-50%, 15 min |
| Buffer | Gradient AmFm pH 3.0- 20-80 mM, 15 min |
| Flow Rate | 1.0 ml/min |
| Detection | UV 280 nm, MS-compatible mobile phase |
| Class of Compounds | Drug, Hydrophilic, Supplements, Monoamine, Neurotransmitter |
| Analyzing Compounds | Serotonin, Tryptophan, N,N-dimethyltryptophan, N,N-Dimethyl-5-methoxytryptamine, Tryptamine, N,N-dimethyltryptamine |
Application Analytes:
N,N-Dimethyl-5-methoxytryptamine
N,N-Dimethyltryptamine
N,N-dimethyltryptophan
Serotonin
Tryptamine
Tryptophan