| CAS Number | 73590-58-6 |
|---|---|
| Molecular Formula | C17H19N3O3S |
| Molecular Weight | 345.421 |
| InChI Key | SUBDBMMJDZJVOS-UHFFFAOYSA-N |
| LogP | 2.23 |
| Synonyms |
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Applications:
Determination Omeprazole on Obelisc R Column
February 11, 2021
Omeprazole is a proton pump inhibitor, a medication that decreases the amount of acid produced in the stomach. Omeprazole has a bactericidal effect on Helicobacter pylori.
Obelisc R column which has both positive and negative ion-pairs embedded in the stationary phase allows for fine tuning and separation of a wide range of compounds with different ionic properties. Omeprazole was separated isocratically using a simple MS-compatible mobile phase of acetonitrile (ACN) and water with Ammonium Acetate (AmAc) buffer. Can also be UV detected at 300nm.
| Column | Obelisc R, 4.6×150 mm, 5 µm, 100A |
| Mobile Phase | MeCN -35% |
| Buffer | Ammonium Acetate pH 5.5 – 10 mM |
| Flow Rate | 1.0 ml/min |
| Detection | UV 300 nm |
| Column | Obelisc R, 4.6×150 mm, 5 µm, 100A |
| Mobile Phase | Gradient MeCN -20-50% in 15 min + 5 min hold |
| Buffer | Ammonium Acetate pH 5.5 – 10 mM |
| Flow Rate | 1.0 ml/min |
| Detection | UV 300 nm |
| Class of Compounds |
Drug |
| Analyzing Compounds | Omeprazole |
Application Column
Obelisc R
SIELC has developed the Obelisc™ columns, which are mixed-mode and utilize Liquid Separation Cell technology (LiSC™). These cost-effective columns are the first of their kind to be commercially available and can replace multiple HPLC columns, including reversed-phase (RP), AQ-type reversed-phase, polar-embedded group RP columns, normal-phase, cation-exchange, anion-exchange, ion-exclusion, and HILIC (Hydrophilic Interaction Liquid Chromatography) columns. By controlling just three orthogonal method parameters - buffer concentration, buffer pH, and organic modifier concentration - users can adjust the column properties with pinpoint precision to separate complex mixtures.
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Separation of Omeprazole on Newcrom R1 HPLC column
February 16, 2018
Omeprazole can be analyzed by this reverse phase (RP) HPLC method with simple conditions. The mobile phase contains an acetonitrile (MeCN), water, and phosphoric acid. For Mass-Spec (MS) compatible applications the phosphoric acid needs to be replaced with formic acid. Smaller 3 µm particles columns available for fast UPLC applications. This liquid chromatography method is scalable and can be used for isolation impurities in preparative separation. It also suitable for pharmacokinetics.
Application Column
Newcrom R1
The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.
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