| CAS Number | 148553-50-8 |
|---|---|
| Molecular Formula | C8H17NO2 |
| Molecular Weight | 159.230 |
| InChI Key | AYXYPKUFHZROOJ-ZETCQYMHSA-N |
| LogP | -0.546 |
| Synonyms |
|
Applications:
HPLC Method for Determination of Gamma-aminobutyric acid (GABA), Vigabatrin, Phenibut, Pregabalin, Baclofen on Primesep 100 Column
June 15, 2021
Separation type: Liquid Chromatography Mixed-mode
High Performance Liquid Chromatography (HPLC) Method for Analysis of Gamma-aminobutyric acid (GABA), Vigabatrin, Phenibut, Pregabalin, Baclofen
All gabapentinoids can be retained and separated on the Primesep 100 mixed-mode column using an isocratic analytical method with a simple mobile phase of water, acetonitrile (MeCN, ACN), and Ammonium Formate (AmFm) buffer. Detection can be achieved with mass spectrometry (MS), evaporative light scattering detection (ELSD) and Charged aerosol detection (CAD).
| Column | Primesep 100, 4.6×150 mm, 5 µm, 100A |
| Mobile Phase | MeCN/H2O – 30/70% |
| Buffer | AmFm pH 3.0 – 50 mM |
| Flow Rate | 1.0 ml/min |
| Detection | CAD (Corona) (MS-compatible mobile phase) |
| Class of Compounds | Acid, Drug |
| Analyzing Compounds | Gamma-aminobutyric acid (GABA), Vigabatrin, Phenibut, Pregabalin, Baclofen |
Application Column
Primesep 100
The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.
Select optionsPhenibut
Pregabalin
Vigabatrin
gamma-Aminobutyric Acid (GABA)
HPLC Determination of Gamma-aminobutyric Acid (GABA), Gabapentin and Pregabalin on Primesep N Column
June 8, 2021
Separation type: Liquid Chromatography Mixed-mode
View on hplc.cloud
High Performance Liquid Chromatography (HPLC) Method for Analysis of GABA, Gabapentin and Pregabalin
| Column | Primesep N, 4.6×150 mm, 5 µm, 100A |
| Mobile Phase | MeCN/H2O |
| Buffer | AmFm pH 3.0 – 10 mM |
| Flow Rate | 1.0 ml/min |
| Detection | CAD (Corona) (MS-compatible mobile phase) |
| Class of Compounds |
Acid, Drug |
| Analyzing Compounds | Gamma-Aminobutyric acid (GABA), Gabapentin, Pregabalin |
Application Column
Primesep N
The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.
Select optionsGabapentin hydrochloride
Pregabalin
gamma-Aminobutyric Acid (GABA)
Separation of Pregabalin on Newcrom R1 HPLC column
February 16, 2018
Pregabalin can be analyzed by this reverse phase (RP) HPLC method with simple conditions. The mobile phase contains an acetonitrile (MeCN), water, and phosphoric acid. For Mass-Spec (MS) compatible applications the phosphoric acid needs to be replaced with formic acid. Smaller 3 µm particles columns available for fast UPLC applications. This liquid chromatography method is scalable and can be used for isolation impurities in preparative separation. It also suitable for pharmacokinetics.
Application Column
Newcrom R1
The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.
Select options