Primesep B

Primesep B is a reverse-phase analytical column with embedded basic ion-pairing groups specifically designed for direct plasma analysis.

It contains a C12 carbon chain and basic groups with  pKa of 10.

Primesep B - Basic

  • Does not retain high-molecular weight plasma components.
  • Retains hydrophobic and acidic components.
  • All mobile phases are LC-MS and preparative chromatography compatible (working pH range from 1.5 to 4).

Primesep D columns are available in all standard dimensions
Column IDs: 22 mm, 10 mm, 4.6 mm, 3.2 mm, 2.1 mm, 1 mm, 0.5 mm, 0.25 mm
Column Lengths: 250 mm, 150 mm, 100 mm, 50 mm, 25 mm, 10 mm
Particles: 10 µm, 5 µm
Pores: 100 A


Primesep B Application Examples

HPLC Determination of β‑Hydroxy-β-methylbutyric Acid

HPLC Separation of Acrylic Acid and 4- Methoxyphenol on Primesep B Column

HPLC Determination of Acrylic Acid on Primesep B Column

HPLC Method for Analysis of Meclizine

HPLC Determination of Cyclopropanecarboxylic Acid