Applications:
HPLC Separation Of Mixture of Pyridylacetic Acids And Their Derivatives
May 23, 2018
HPLC Method for 2-Pyridylacetic acid hydrochloride, 3-Pyridylacetic acid hydrochloride, 4-Pyridylacetic acid hydrochloride, Nicotinic Acid/Niacin (3-pyridinecarboxylic acid), 3-Acetylpyridine, Pyridyl-3-thioacetomorpholide on Primesep 200 by SIELC Technologies
High Performance Liquid Chromatography (HPLC) Method for Analysis of 2-Pyridylacetic acid hydrochloride, 3-Pyridylacetic acid hydrochloride, 4-Pyridylacetic acid hydrochloride, Nicotinic Acid/Niacin (3-pyridinecarboxylic acid), 3-Acetylpyridine, Pyridyl-3-thioacetomorpholide
Pyridylacetic Acids are a class of compounds with a pyridine ring and an acetic acid. These acids are typically building blocks found in organic synthesis/ On occasion, they can also act as human xenobiotic metabolites.
Nicotinic acid, also known as niacin and vitamin B3, is a water-soluble B vitamin with C₆H₅NO₂ molecular formula. It is crucial for energy production.
Derrivatives are compounds formed when the parent compound goes through a chemical recation, replacing atoms with different ones, leading to different physical and chemical properties.
2-Pyridylacetic acid hydrochloride, 3-Pyridylacetic acid hydrochloride, 4-Pyridylacetic acid hydrochloride, Nicotinic Acid/Niacin (3-pyridinecarboxylic acid), 3-Acetylpyridine, Pyridyl-3-thioacetomorpholide can be retained and analyzed using the Primesep 200 stationary phase column. The analysis utilizes a gradient method with a simple mobile phase consisting of water and acetonitrile (MeCN) with a formic acid buffer. Detection is performed using UV.
| Column | Primesep 200, 4.6 x 250 mm, 5 µm, 100 A, dual ended | 
| Mobile Phase | MeCN/H2O Gradient – 5-50% | 
| Buffer | CH2O2 – 0.02-0.05% | 
| Flow Rate | 1.0ml/min | 
| Detection | UV, 270 nm | 
| Class of Compounds | Acids | 
| Analyzing Compounds | 2-Pyridylacetic acid hydrochloride, 3-Pyridylacetic acid hydrochloride, 4-Pyridylacetic acid hydrochloride, Nicotinic Acid/Niacin (3-pyridinecarboxylic acid), 3-Acetylpyridine, Pyridyl-3-thioacetomorpholide | 
Application Column
Primesep 200
Column Diameter: 4.6 mm
Column Length: 250 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended
3-Acetylpyridine
3-Pyridylacetic acid hydrochloride
4-Pyridylacetic acid hydrochloride
Nicotinic Acid/Niacin (3-pyridinecarboxylic acid)
Pyridyl-3-thioacetomorpholide


