Quercetin dihydrate

Quercetin dihydrate structural formula

CAS Number6151-25-3
Molecular FormulaC15H14O9
Molecular Weight338.269
InChI KeyGMGIWEZSKCNYSW-UHFFFAOYSA-N
LogP1.57
Synonyms
  • Quercetin dihydrate
  • 2-(3,4-Dihydroxyphenyl)-3,5,7-trihydroxy-4H-1-benzopyran-4-one--water (1/2)
  • 4H-1-Benzopyran-4-one, 2-(3,4-dihydroxyphenyl)-3,5,7-trihydroxy-, hydrate (1:2)
  • 6151-25-3
  • UNII-53B03V78A6

Applications:

HPLC Separation of Cinnamic Acid, Salicylic Acid and Quercetin on Newcrom B Column

March 12, 2021

Separation type: Liquid Chromatography Mixed-mode




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High Performance Liquid Chromatography (HPLC) Method of Cinnamic Acid, Salicylic Acid and Quercetin.

Cinnamic acid and benzoic acid are two of the major phenolic acids and autotoxins. Cinnamic acid occurs naturally in all green plants, showing significant antimicrobial activity against both bacteria and fungi as well as antioxidant activity. Salicylic acid (SA) is an o-hydroxybenzoic acid, synthesized by both plants and microorganisms. SA acts as a critical plant hormone regulating various processes, including growth and development, flowering, thermogenesis, ion uptake, stomatal movement, photosynthesis, and plant immunity. Quercetin is a naturally occurring flavonol, or flavonoid, the yellowish antioxidant pigment found in skins of red grapes, berries, apples, onions, tomatoes, and buckwheat tea. In addition to functioning as a flavonoid, quercetin is also a phytoestrogen. All three compounds can be retained and separated by using a mixed-mode Newcrom B column using an isocratic analytical method with a simple mobile phase of water, acetonitrile (MeCN, ACN), and phosphoric acid (H3PO4) buffer. UV detection at 250 nm.



Condition

Column Newcrom B, 4.6×150 mm, 5 µm, 100A
Mobile Phase MeCN -50%
Buffer H3PO4
Flow Rate 1.0 ml/min
Detection 250 nm

Description

Class of Compounds Acid
Analyzing Compounds Cinnamic acid, Quercetin, Salicylic acid

 

Application Column

Newcrom B

The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.

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Application Analytes:
Cinnamic acid
Quercetin
Quercetin dihydrate
Salicylic acid
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

HPLC Separation of Phenolic Acids

March 11, 2021


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Condition 1

Column Newcrom B, 4.6×150 mm, 5 µm, 100A
Mobile Phase Gradient MeCN
Buffer H3PO4 – 0.2%
Flow Rate 1.0 ml/min
Detection 270 nm

Condition 2

Column Newcrom R1, 4.6×150 mm, 5 µm, 100A
Mobile Phase Gradient MeCN
Buffer H3PO4 – 0.2%
Flow Rate 1.0 ml/min
Detection 270 nm

Description

Class of Compounds Acid
Analyzing Compounds Gallic acid, 3,4 Dihydroxybenzoic acid, Caffeic acid, trans-Ferulic acid, trans- p -Coumaric acid, trans-Cinnamic acid, Quercetin Hydrate, Salicylic acid

 

Application Column

Newcrom B

The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.

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Newcrom R1

The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.

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Application Analytes:
3,4-Dihydroxybenzoic Acid
Caffeic acid
Cinnamic acid
Ferulic acid
Gallic acid
Quercetin
Quercetin dihydrate
Salicylic acid
p-coumaric acid
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

Separation of Quercetin dihydrate on Newcrom R1 HPLC column

May 16, 2018
Separation of Quercetin dihydrate on Newcrom R1 HPLC column

Quercetin dihydrate is a flavonol found in many plants. It has a bitter flavor and is a polar auxin transport inhibitor. Quercetin can be analyzed by this reverse phase (RP) HPLC method with simple conditions. The mobile phase contains acetonitrile (MeCN), water, and phosphoric acid. For Mass-Spec (MS) compatible applications the phosphoric acid needs to be replaced with formic acid. Smaller 3 µm particle columns available for fast UPLC applications. This liquid chromatography method is scalable and can be used for isolation of impurities in preparative separation. It also suitable for pharmacokinetics.

Application Column

Newcrom R1

The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.

Select options
Application Analytes:
Quercetin dihydrate
The result was obtained by a proprietary SIELC algorithm. It may deviate from the actual experimental data. The experimental data are available upon request. Contact us by e-mail: support@sielc.com or by phone: 847-229-2629.