|Molecular Weight||337.840 g/mol|
Ractopamine hydrochloride can be analyzed by this reverse phase (RP) HPLC method with simple conditions. The mobile phase contains an acetonitrile (MeCN), water, and phosphoric acid. For Mass-Spec (MS) compatible applications the phosphoric acid needs to be replaced with formic acid. Smaller 3 µm particles columns available for fast UPLC applications. This liquid chromatography method is scalable and can be used for isolation impurities in preparative separation. It also suitable for pharmacokinetics.
Ractopamine is a livestock feed additive designed to promote muscle synthesis and leanness. It is banned in many countries, excluding the US, Canada and Mexico. It can be retained on a Primesep 100 Column in about 7 minutes. Primesep 100 is a reverse-phase (RP) mixed-mode column with embedded acidic ion-pairing groups.
|Column||Primesep 100, 4.6x150 mm, 5 µm, 100A|
|Mobile Phase||MeCN/H2O - 20/80%|
|Buffer||H2SO4 - 0.3 %|
|Flow Rate||1.0 ml/min|
|Detection||UV, 222 nm|
|Class of Compounds||Drug, Basic, Hydrophobic, Ionizable, Amine|