Resazurin

CAS Number 550-82-3
Molecular Formula C12H7NO4
Molecular Weight 229.191 g/mol
InChI Key PLXBWHJQWKZRKG-UHFFFAOYSA-N
LogP 0.806
Synonyms
  • Resazurin
  • 7-Hydroxy-10-oxo-3H-10lambda5-phenoxazin-3-one
  • 3H-Phenoxazin-3-one, 7-hydroxy-, 10-oxide
  • 550-82-3
  • 3H-?Phenoxazin-?3-?one, 7-?hydroxy-?, 10-?oxide
  • 7-Hydroxy-10-oxo-3H-10 lambda5-phenoxazin-3-one
  • 7-Hydroxy-3H-phenoxazin-3-one 10-oxide
  • 7-Hydroxy-3H-phenoxazine-3-one 10-oxide
  • Alamar Blue
  • NSC 10438
  • NSC 56323
  • Rhodalux
  • 10-Oxyde de 7-hydroxy-3H-phenoxazin-3-one
  • 10-oxyde de 7-hydroxy-3H-phenoxazine-3-one
  • 7-Hydroxy-3H-phenoxazin-3-on-10-oxid
  • 10-oxido de 7-hidroxi-3H-fenoxazin-3-on
  • 4-27-00-03594
  • Azoresorcin
  • BRN 0221382
  • Diazoresorcinol
  • EINECS 208-987-1
  • Resazoin
  • Resazurine
  • 7-Hydroxy-3H-fenoxazin-3-on-10-oxid
  • UNII-1FN9YD6968
  • 153796-08-8

Applications:


Separation of Resazurin on Newcrom R1 HPLC column

Resazurin can be analyzed by this reverse phase (RP) HPLC method with simple conditions. The mobile phase contains an acetonitrile (MeCN), water, and phosphoric acid. For Mass-Spec (MS) compatible applications the phosphoric acid needs to be replaced with formic acid. Smaller 3 µm particles columns available for fast UPLC applications. This liquid chromatography method is scalable and can be used for isolation impurities in preparative separation. It also suitable for pharmacokinetics.

Separation of Resazurin on Newcrom C18 HPLC column
Newcrom R1

This column is available in new type of hardware with only one connection end. Learn more about performance and advantage of this new hardware.



Application Analytes:

Resazurin

HPLC Method for Analysis of Dyes
HPLC Separation of Dyes

3-Aminoacridine is an aminoacridine. Alizarin a red pigment that comes as a red powder with a slight orange tint. When dissolved it appears bright red with a slight pink undertone. Alizarin is also known as Mordant Red 11 or Turkey Red. Eosin Y is a pigment that comes as an orange powder that when dissolved in water turns bright orange. Eosin is a fluorescent staining agent. It is used to stain red blood cells, proteins on cytoplasm, collage, and muscle fibers. It is also fluorescence. Fluorescein is a dye that comes in a dark orange powder and is used as a tracer and an indicator. As it says in the name, it is also Fluorescent, obtaining a green-yellow color when under black light. Methyl red an indicator that turns red in acidic solutions (below 4.4 pH), is a shade of orange between 4.5 and 6.1 pH, and turns bright yellow in basic solutions (above pH 6.2). Nile blue, also known as Nile blue A, is used as a stain used in biology and histology as well as a pH indicator. In pH below 7 it becomes lighter shades of glue, and at pH 0 becomes almost completely translucent. At pH of seven it has a dark blue shade, while at pH higher than that it starts becoming more red. Pararosaniline Hydrochloride, also known as Basic Red 9, or C.I. 42500. It is a a magenta solid that is used as a dye. Patent Blue Violet, also known as Patent Blue, is a deep blue pigment that is often used to dye clothes. Resazurin is a blue dye as well as a pH indicator. At pH 3.8 or lower, it is bright orange, but the higher the pH the more blue it becomes, the color stops changing at the pH of 6.5 where it becomes indigo. Newcrom R1, a column that takes advantage of the newest technologies, does not contain embedded acidic nor basic ionizable groups and can retain dyes. The method is UV compatible and can be used as a general approach for analyzing similar compounds.

Application Analytes:

3-Aminoacridine
Alizarin
Carmine
Eosin Y
Fluorescein
Methyl Red
Nile Blue A
Pararosaniline Hydrochloride
Patent Blue Violet (Patent Blue)
Resazurin