Streptomycin B

Streptomycin B structural formula

CAS Number128-45-0
Molecular FormulaC27H49N7O17
Molecular Weight743.722
InChI KeyHQXKELRFXWJXNP-FJYWXVICSA-N
LogP-4.92
Synonyms
  • Streptomycin B
  • N,N'-[(1S,2R,3R,4S,5R,6R)-2,4,5-Trihydroxy-6-{[beta-D-mannopyranosyl-(1->4)-2-deoxy-2-(methylamino)-alpha-L-glucopyranosyl-(1->2)-5-deoxy-3-C-formyl-alpha-L-lyxofuranosyl]oxy}cyclohexane-1,3-diyl]diguanidine
  • Guanidine, N,N'''-[(1S,2R,3R,4S,5R,6R)-2,4,5-trihydroxy-6-[[O-beta-D-mannopyranosyl-(1->4)-O-2-deoxy-2-(methylamino)-alpha-L-glucopyranosyl-(1->2)-5-deoxy-3-C-formyl-alpha-L-lyxofuranosyl]oxy]-1,3-cyclohexanediyl]bis-
  • 128-45-0
  • 4-18-00-07546
  • BRN 0077587
  • EINECS 204-887-7
  • Mannosidostreptomycin
  • UNII-CJ1467C885
  • 11031-73-5
  • 26215-55-4
  • 47879-98-1
  • 744950-19-4

Applications:

HPLC Determination of Streptomycin on Primesep 200 Column

May 9, 2019

HPLC Method for Streptothricins, Streptomycin B, Streptomycin on Primesep 200 by SIELC Technologies

HPLC Determination of Streptomycin sulfate salt on Primesep 200 Column

High Performance Liquid Chromatography (HPLC) Method for Analysis of Streptothricins, Streptomycin B, Streptomycin.

Streptomycin is an antibiotic used in treating bacterial infections and has also been used as a pesticide. It is on the World Health Organization’s (WHO) list of essential medicines. It works by inhibiting the synthesis of proteins. It can be retained on a Primesep 200 mixed-mode column by hydrophobic and ion-exchange properties. The analytical method uses MS-compatible mobile phase of acetonitrile (ACN) and water with sulfuric acid (H2SO4) buffer. Also capable of UV detection at 200nm.

Condition
Column Primesep 200
Mobile Phase MeCN/H2O – 10/90%
Buffer H2SO4 – 0.05%
Flow Rate 1.0 ml/min
Detection UV, 200 nm

 

Description
Class of Compounds
 Drug, Hydrophilic, Zwitterionic, Antibiotic
Analyzing Compounds Streptothricins, Streptomycin B, Streptomycin

 

Application Column

Primesep 200

Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended

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Application Analytes:
Streptomycin
Streptomycin B
Streptothricins
Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

Separation of Streptomycin B on Newcrom R1 HPLC column

May 16, 2018
Separation of Streptomycin B on Newcrom R1 HPLC column

Streptomycin B can be analyzed by this reverse phase (RP) HPLC method with simple conditions. The mobile phase contains an acetonitrile (MeCN), water, and phosphoric acid. For Mass-Spec (MS) compatible applications the phosphoric acid needs to be replaced with formic acid. Smaller 3 µm particles columns available for fast UPLC applications. This liquid chromatography method is scalable and can be used for isolation impurities in preparative separation. It also suitable for pharmacokinetics.

Application Column

Newcrom R1

The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.

Select options
Application Analytes:
Streptomycin B
The result was obtained by a proprietary SIELC algorithm. It may deviate from the actual experimental data. The experimental data are available upon request. Contact us before ordering the column as there may be a more suitable column alternative by either e-mail: support@sielc.com or by phone: 847-229-2629.