Streptomycin B

CAS Number 128-45-0
Molecular Formula C27H49N7O17
Molecular Weight 743.722 g/mol
LogP -4.92
  • Streptomycin B
  • N,N'-[(1S,2R,3R,4S,5R,6R)-2,4,5-Trihydroxy-6-{[beta-D-mannopyranosyl-(1->4)-2-deoxy-2-(methylamino)-alpha-L-glucopyranosyl-(1->2)-5-deoxy-3-C-formyl-alpha-L-lyxofuranosyl]oxy}cyclohexane-1,3-diyl]diguanidine
  • Guanidine, N,N'''-[(1S,2R,3R,4S,5R,6R)-2,4,5-trihydroxy-6-[[O-beta-D-mannopyranosyl-(1->4)-O-2-deoxy-2-(methylamino)-alpha-L-glucopyranosyl-(1->2)-5-deoxy-3-C-formyl-alpha-L-lyxofuranosyl]oxy]-1,3-cyclohexanediyl]bis-
  • 128-45-0
  • 4-18-00-07546
  • BRN 0077587
  • EINECS 204-887-7
  • Mannosidostreptomycin
  • UNII-CJ1467C885
  • 11031-73-5
  • 26215-55-4
  • 47879-98-1
  • 744950-19-4


Separation of Streptomycin B on Newcrom R1 HPLC column

Streptomycin B can be analyzed by this reverse phase (RP) HPLC method with simple conditions. The mobile phase contains an acetonitrile (MeCN), water, and phosphoric acid. For Mass-Spec (MS) compatible applications the phosphoric acid needs to be replaced with formic acid. Smaller 3 µm particles columns available for fast UPLC applications. This liquid chromatography method is scalable and can be used for isolation impurities in preparative separation. It also suitable for pharmacokinetics.

Separation of Streptomycin B on Newcrom R1 HPLC column
Newcrom R1

This column is available in new type of hardware with only one connection end. Learn more about performance and advantage of this new hardware.

Application Analytes:

Streptomycin B

HPLC Determination of Streptomycin on Primesep 200 Column
HPLC Determination of Streptomycin sulfate salt on Primesep 200 Column  


Column Primesep 200, 4.6x150 mm, 5 µm, 100A
Mobile Phase MeCN/H2O - 10/90%
Buffer H2SO4 - 0.05%
Flow Rate 1.0 ml/min
Detection UV, 200 nm


Class of Compounds Drug, Hydrophilic, Zwitterionic, Antibiotic
Analyzing Compounds Streptomycin

Application Analytes:

Streptomycin B