Buffer Recommendation

Reported concentration of acid in g/L always

Physical Properties of Acids Useful in HPLC Application

AcidConcentrationDensity g/mL1 Molarity in g/molGrams of Acid to get 1% in g/LmL of acid to get 1% in g/L 
Phosphoric acid 75%1.589813.38.44
Sulfuric acid 981.839810.25.58
TFA1001.49114106.71
Formic acid1001.2246108.20
Acetic acid99.81.0560109.52
Perchloric acid701.6610014.38.61

Buffer pH range UV range, nm MS, ELSD

TFA 1.5-2.5 >210 + Acetic acid 3.5-5.0 > 220 ++ Formic acid 2.5 4.0 > 220 ++ H2SO4 1.0-2.5 > 195 +- HClO4 1.5-2.5 > 195 – H3PO4 1.5-3.0 > 195 — TEA phosphate 1.8-3.1 > 195 — Sodium Phosphate 1.8-3.1 > 195 — 6.0-7.0 > 195 — Ammonium Phosphate 1.8-3.1 > 195 — TEA Formate 2.5-4.0 > 220 + TEA Acetate 3.5-5.0 > 220 + Ammonium Formate 2.5-4.0 > 220 ++ Ammonium Acetate 3.5-5.0 > 220 ++