Thymol

Thymol structural formula

CAS Number89-83-8
Molecular FormulaC10H14O
Molecular Weight150.221
InChI KeyMGSRCZKZVOBKFT-UHFFFAOYSA-N
LogP3.30
Synonyms
  • Thymol
  • 5-Methyl-2-(propan-2-yl)phenol
  • Phenol, 5-methyl-2-(1-methylethyl)-
  • 89-83-8
  • Phenol, 5-methyl-2-(1-methylethyl)-
  • 1-Methyl-3-hydroxy-4-isopropylbenzene
  • 2-Hydroxy-1-isopropyl-4-methylbenzene
  • 2-ISOPROPYL-5-METHYLPHENOL
  • 3-Hydroxy-p-cymene
  • 3-Methyl-6-isopropylphenol
  • 5-Methyl-2-(1-methylethyl)phenol
  • 5-Methyl-2-isopropyl-1-phenol
  • 5-Methyl-2-isopropylphenol
  • 6-Isopropyl-3-methylphenol
  • 6-Isopropyl-m-cresol
  • Apiguard
  • m-Thymol
  • NSC 11215
  • NSC 47821
  • NSC 49142
  • p-Cymen-3-ol
  • Thyme camphor
  • Thymol Chrystals
  • Caswell No. 856A
  • m-Cresol, 6-isopropyl-
  • p-Cymene, 3-hydroxy-
  • Cymophenol, alpha-
  • EINECS 201-944-8
  • EPA Pesticide Chemical Code 080402
  • FEMA No. 3066
  • FEMA Number 3066
  • Phenol, 2-isopropyl-5-methyl-
  • UNII-3J50XA376E
  • 1-hydroxy-5-methyl-2-isopropylbenzene
  • 3-Hydroxy-1-methyl-4-isopropylbenzene
  • 3-Hydroxy-p-cymene
  • 3-p-cymenol
  • 6-Isopropyl-p-cresol
  • Isopropyl cresol
  • Isopropyl-m-cresol
  • Thymate
  • Thymic acid
  • Thymol crystal puriss

Applications:

HPLC Determination of Thymol and Carvacrol on Primesep B Column

July 14, 2021

Separation type: Liquid Chromatography Mixed-mode




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High Performance Liquid Chromatography (HPLC) Method for Analysis of Thymol and Carvacrol.



Thymol (2-isopropyl-5-methylphenol) and Carvacrol (5-Isopropyl-2-methylphenol) are the main components of the essential oils of the Lamiaceae family of plants. Thymol and carvacrol can also be applied as an alternative antimicrobial agent against antibiotic-resistant pathogenic bacteria. Thymol and Carvacrol can be retained in HPLC using Primesep B column with a simple isocratic method using a mobile phase consisting of acetonitrile (MeCN), water and phosphoric acid buffer. The analysis method can be UV detected at 200 nm.




Condition

Column Primesep B, 3.2×100 mm, 5 µm, 100A
Mobile Phase MeCN/H2O – 45/65%
Buffer H3PO4 – 0.1%
Flow Rate 0.5 ml/min
Detection 200 nm

Description

Class of Compounds Phenol, Hydrophobic
Analyzing Compounds Thymol, carvacrol

 

Application Column

Primesep B

The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.

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Application Analytes:
Carvacrol
Thymol
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

HPLC Determination of Thymol on Newcrom R1

June 14, 2021

Separation type: Liquid Chromatography Reverse Phase.




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High Performance Liquid Chromatography (HPLC) Method for Analysis of Thymol.




Thymol (2-isopropyl-5-methylphenol) is a natural monoterpenoid phenol derivative of p-Cymene. Thymol is the main monoterpene phenol occurring in essential oils isolated from plants such as the Thymus, Ocimum, Origanum, and other herbs with antimicrobial properties. Thymol can be retained in HPLC using Newcrom R1 column with the simple isocratic mobile phase consisting of methanol (MeOH) and water. The analysis method can be UV detected at 210nm.




 

Condition

Column Newcrom R1, 2.1×100 mm, 5 µm, 100A
Mobile Phase MeOH – 70%
Buffer No
Flow Rate 0.2 ml/min
Detection 210 nm

Description

Class of Compounds Phenol, Hydrophobic
Analyzing Compounds Thymol

 

Application Column

Newcrom R1

The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.

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Application Analytes:
Thymol
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

Separation of Thymol on Newcrom R1 HPLC column

February 16, 2018
Separation of Thymol on Newcrom C18 HPLC column

Thymol can be analyzed by this reverse phase (RP) HPLC method with simple conditions. The mobile phase contains an acetonitrile (MeCN), water, and phosphoric acid. For Mass-Spec (MS) compatible applications the phosphoric acid needs to be replaced with formic acid. Smaller 3 µm particles columns available for fast UPLC applications. This liquid chromatography method is scalable and can be used for isolation impurities in preparative separation. It also suitable for pharmacokinetics.

Application Column

Newcrom R1

The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.

Select options
Application Analytes:
Thymol
The result was obtained by a proprietary SIELC algorithm. It may deviate from the actual experimental data. The experimental data are available upon request. Contact us by e-mail: support@sielc.com or by phone: 847-229-2629.