Trimetazidine

Trimetazidine structural formula

CAS Number5011-34-7
Molecular FormulaC14H22N2O3
Molecular Weight266.342
InChI KeyUHWVSEOVJBQKBE-UHFFFAOYSA-N
LogP0.890
Synonyms
  • Trimetazidine
  • 1-[(2,3,4-Trimethoxyphenyl)methyl]piperazine
  • Piperazine, 1-[(2,3,4-trimethoxyphenyl)methyl]-
  • 5011-34-7
  • EINECS 225-690-2
  • Trimetazidina
  • Trimetazidinum
  • UNII-N9A0A0R9S8

Applications:

HPLC Method For Analysis Of Trimetazidine Hydrochloride on Primesep 100 Column

March 22, 2022

HPLC Method for Trimetazidine on Primesep 100 by SIELC Technologies

 

High Performance Liquid Chromatography (HPLC) Method for Analysis of Trimetazidine.

Trimetazidine (TMZ) is a medication with the chemical formula C14H22N2O3. It is taken to treat angina and other heart conditions. It inhibits the mitochondrial enzyme long-chain-3-ketoacyl-CoA thiolase  which decreases fatty acids and shifts substrate utilization towards glucose oxidation. There is a debate within the sports and scientific communities about whether or not TMZ is a performance-enhancing drug. The World Anti-Doping Agency considers it to be a hormone and metabolic modulator.

Trimetazidine can be retained and analyzed using the Primesep 100 stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with a sulfuric acid buffer. Detection is performed using UV.

Condition
Column Primesep 100, 4.6 x 150 mm, 5 µm, 100 A, dual ended
Mobile Phase MeCN/H2O – 60/40%
Buffer H2SO4 – 0.4%
Flow Rate 1.0 ml/min
Detection UV, 200 nm,

 

Description
Class of Compounds
 Drug
Analyzing Compounds Trimetazidine

 

Application Column

Primesep 100

Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended

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Application Analytes:
Trimetazidine
Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

Separation of Trimetazidine on Newcrom R1 HPLC column

February 16, 2018
Separation of Trimetazidine on Newcrom C18 HPLC column

Trimetazidine can be analyzed by this reverse phase (RP) HPLC method with simple conditions. The mobile phase contains an acetonitrile (MeCN), water, and phosphoric acid. For Mass-Spec (MS) compatible applications the phosphoric acid needs to be replaced with formic acid. Smaller 3 µm particles columns available for fast UPLC applications. This liquid chromatography method is scalable and can be used for isolation impurities in preparative separation. It also suitable for pharmacokinetics.

Application Column

Newcrom R1

The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.

Select options
Application Analytes:
Trimetazidine
The result was obtained by a proprietary SIELC algorithm. It may deviate from the actual experimental data. The experimental data are available upon request. Contact us before ordering the column as there may be a more suitable column alternative by either e-mail: support@sielc.com or by phone: 847-229-2629.