Trimethoprim

Trimethoprim structural formula

CAS Number738-70-5
Molecular FormulaC14H18N4O3
Molecular Weight290.323
InChI KeyIEDVJHCEMCRBQM-UHFFFAOYSA-N
LogP0.91
Synonyms
  • Trimethoprim
  • 5-[(3,4,5-Trimethoxyphenyl)methyl]pyrimidine-2,4-diamine
  • 2,4-Pyrimidinediamine, 5-[(3,4,5-trimethoxyphenyl)methyl]-
  • 738-70-5
  • 5-25-13-00429
  • 2,4-Pyrimidinediamine, 5-[(3,4,5-trimethoxyphenyl)methyl]-
  • 2,4-Diamino-5-(3',4',5'-trimethoxybenzyl)pyrimidine
  • 2,4-Diamino-5-(3,4,5-trimethoxybenzyl)pyrimidine
  • 5-[(3,4,5-Trimethoxyphenyl)methyl]-2,4-pyrimidinediamine
  • Instalac
  • Monotrim
  • NSC 106568
  • Proloprim
  • Pyrimidine, 2,4-diamino-5-(3,4,5-trimethoxybenzyl)-
  • Syraprim
  • Trimanyl
  • Trimethioprim
  • trimethoprime
  • Trimetoprim
  • trimetoprima
  • Trimogal
  • Trimopan
  • Trimpex
  • Triprim
  • Uretrim
  • Wellcoprim
  • BRN 0625127
  • EINECS 212-006-2
  • Monoprim
  • Monotrimin
  • NIH 204
  • NSC-106568
  • 2,4-Pyrimidinediamine, 5-((3,4,5-trimethoxyphenyl)-methyl)-
  • 5-(3,4,5-Trimethoxybenzyl)-2,4-diaminopyrimidine
  • 5-((3,4,5-Trimethoxyphenyl)methyl)-2,4-pyrimidinediamine
  • component of Septra
  • component of Bactrim
  • Abaprim
  • Alcorim-F
  • Antrima
  • Chemotrin
  • Dosulfin
  • Duocide
  • Kombinax
  • Metoprim
  • Pancidim
  • Protrin
  • Sinotrim
  • Streptoplus
  • Trimexol
  • Trimez-IFSA
  • Trimono
  • Utetrin
  • Velaten
  • Wellcoprin
  • Zamboprim
  • Trimpex 200
  • Trimethoprimum
  • UNII-AN164J8Y0X

Applications:

HPLC Method For Analysis Of Sulfamethoxazole and Trimethoprim on Primesep 100 Column

March 22, 2022

Separation type: Liquid Chromatography Mixed-mode


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High Performance Liquid Chromatography (HPLC) Method for Analysis of Sulfamethoxazole and Trimethoprim

Condition
Column Primesep 100, Newcrom R1 4.6×150 mm, 5 µm, 100A
Mobile Phase MeCN/H2O – 40/60%
Buffer H2SO4 – 0.4%
Flow Rate 1.0 ml/min
Detection UV, 200 nm,

 

Description
Class of Compounds
 Drug
Analyzing Compounds Sulfamethoxazole, Trimethoprim

 

Application Column

Newcrom R1

The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.

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Primesep 100

The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.

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Application Analytes:

Sulfamethoxazole
Trimethoprim
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

Separation of Trimethoprim on Newcrom R1 HPLC column

February 16, 2018
Separation of Trimethoprim on Newcrom C18 HPLC column

Trimethoprim can be analyzed by this reverse phase (RP) HPLC method with simple conditions. The mobile phase contains an acetonitrile (MeCN), water, and phosphoric acid. For Mass-Spec (MS) compatible applications the phosphoric acid needs to be replaced with formic acid. Smaller 3 µm particles columns available for fast UPLC applications. This liquid chromatography method is scalable and can be used for isolation impurities in preparative separation. It also suitable for pharmacokinetics.

Application Column

Newcrom R1

The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.

Select options
Application Analytes:
Trimethoprim
The result was obtained by a proprietary SIELC algorithm. It may deviate from the actual experimental data. The experimental data are available upon request. Contact us by e-mail: support@sielc.com or by phone: 847-229-2629.