Ubiquinol

Molecular FormulaC59H92O4
Molecular Weight865.4
InChI KeyQNTNKSLOFHEFPK-UPTCCGCDSA-N
Synonyms
  • reduced coenzyme Q10
  • 992-78-9
  • ubiquinol-10
  • UNII-M9NL0C577Y
  • CoQ10H2
  • M9NL0C577Y
  • Dihydrocoenzyme Q10
  • 2-[(2E,6E,10E,14E,18E,22E,26E,30E,34E)-3,7,11,15,19,23,27,31,35,39-decamethyltetraconta-2,6,10,14,18,22,26,30,34,38-decaen-1-yl]-5,6-dimethoxy-3-methylbenzene-1,4-diol
  • Ubiquinol [WHO-DD]

Applications:

HPLC Determination of Coenzyme Q10 and Ubiquinol on Primesep D Column

February 11, 2020


Coenzyme Q10, aka ubiquinone, is an enzyme found in animals and is used as a dietary supplement. Ubiquinol is a reduced form of coenzyme Q10. The two compounds are similar in structure and can be difficult to separate in reverse-phase HPLC. Using mixed-mode Primesep D column and the mobile phase with acetonitrile (ACN) and water, without the need for buffer, CoQ10 and ubiquinol can be isocratically separated and UV detected at 275nm.

Condition
Column Primesep D
Mobile Phase MeCN/H2O – 95/5%
Buffer No
Flow Rate 1.0 ml/min
Detection UV 275 nm, Vis 435 nm

 

Description
Class of Compounds Hydrophobic
Analyzing Compounds Coenzyme Q10,  Ubiquinol

Application Column

Primesep D

The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.

Select options
Application Analytes:
Coenzyme q10
Ubiquinol
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.