USP Methods for the Analysis Ketoprofen using the Legacy L1 Column

HPLC Method for Ketoprofen on Legacy L1 by SIELC Technologies

USP Methods for the Analysis Ketoprofen using the Legacy L1 Column

High Performance Liquid Chromatography (HPLC) Method for Analysis of Ketoprofen.

Ketoprofen is a prescription anti-inflammatory drug (NSAID) that is used to treat pain, inflammation, and fever.  It is also used in veterinary medicine. It works through inhibiting cyclooxygenase enzymes, which prevents the synthesis of prostaglandins. It can be administered through capsules, topical gel, injections, and patches. It has the chemical formula C16H14O3.

Ketoprofen can be retained and analyzed using the Legacy L1 stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with a sulfuric acid buffer. Detection is performed using UV.

Condition
Column Legacy L1, 4.6 x 150 mm, 5 µm, 100 A, dual ended
Mobile Phase MeCN/H2O – 43/55%
Buffer NaHPO4 pH3.5 – 2%
Flow Rate 1.0 ml/min
Detection UV, 233 nm

 

Description
Class of Compounds
 Drug, Acid, Ionizable,  Hydrophobic
Analyzing Compounds Ketoprofen

 

Application Column

SIELC Legacy L1 HPLC column

Legacy L1

Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended

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Application Analytes:
Ketoprofen
Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.